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SPAG9 polyclonal antibody

CAT: 0384-BS60271-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS60271-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
JIP-4 (C-jun-amino-terminal kinase-interacting protein 4, Mitogen-activated protein kinase 8-interacting protein 4, Sunday driver 1) is a 1,321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
C-Jun-amino-terminal kinase-interacting protein 4; JIP-4; JNK-interacting protein 4; Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1; SPAG9; HSS, KIAA0516, MAPK8IP4, SYD1; HLC6
Swiss Prot
O60271
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human SPAG9.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 146 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SPAG9 polyclonal antibody detects endogenous levels of SPAG9 protein.
Applications Notes
Western blot (WB) analysis of SPAG9 pAb at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:EC9706 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug)
Host or Source
Rabbit

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