GPR176 polyclonal antibody

G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR176 (G protein-coupled receptor 176), also known as HB-954, GPR or Gm1012, is a 515 amino acid multi-pass membrane protein belonging to the G-protein coupled receptor 1 family. Expressed in brain and spleen, with trace expression in kidney, GPR176 functions as an orphan receptor that is thought to play a role in signaling events throughout the cell. Containing four N-glycosylation sites, seven transmembrane domains and a large C-terminal cytosolic domain, GPR176 is encoded by a gene mapping to human chromosome 15q14.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Probable G-protein coupled receptor 176; HB-954; HB954

Q14439

Human, Mouse, Rat

Synthetic peptide, corresponding to amino acids 470-515 of Human GPR176.

Unconjugated

WB IF

WB: 1:500~1:1000 IF: 1:50~1:200

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 57 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

GPR176 polyclonal antibody detects endogenous levels of GPR176 protein.

Western blot (WB) analysis of GPR176 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:Rat liver tissue lysate