GPR12 polyclonal antibody

• Background :

  G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation) . GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR12 is a 334 amino acid peptide that is expressed primarily in brain, particularly in regions where neuronal differentiation takes place. GPR12 is coupled to an inhibitory G protein. It positively influences differentiation and maturation of post-mitotic neurons, and it may promote the growth of neuronal precursor cells.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  G-protein coupled receptor 12

• Swiss Prot :

  P47775

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 51-96 of Human GPR12.

• Conjugation :

  Unconjugated

• Applications :

  WB IF

• Dilution :

  WB: 1:500~1:1000 IF: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 36 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  GPR12 polyclonal antibody detects endogenous levels of GPR12 protein.

• Applications Notes :

  Western blot (WB) analysis of GPR12 polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat heart tissue lysate

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4