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CYP2A7 polyclonal antibody

CAT: 0384-BS5683-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS5683-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze reactions involved in both drug metabolism and in the synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies, such as CYP1A and CYP2A, based on their sequence similarities. CYP2A7 (cytochrome P450 2A7), also known as CPA7, CPAD, CYPIIA7 or P450-IIA4, is a 494 amino acid peripheral membrane protein that belongs to the CYP2A subfamily of cytochrome P450 proteins. Localized to the endoplasmic reticulum, CYP2A7 can be induced to high levels in liver in response to foreign compounds, such as carcinogens, pesticides and drugs. CYP2A7 functions as a hydroxylase that uses a heme group to catalyze the oxidation of target flavoproteins.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cytochrome P450 2A7; CYPIIA7; Cytochrome P450 IIA4
Swiss Prot
P20853
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 435-480 of Human CYP2A7.
Conjugation
Unconjugated
Applications
WB IHC ICC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 56 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CYP2A7 polyclonal antibody detects endogenous levels of CYP2A7 protein.
Applications Notes
Western blot (WB) analysis of CYP2A7 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate
Host or Source
Rabbit

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