PAK2 (R186) polyclonal antibody

• Background:

  Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK-2p27; PAK-2p34; C-t-PAK2; PAK2; PAKγ

• Swiss Prot:

  Q13177

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 150-200 of Human PAK2.

• Conjugation:

  Unconjugated

• Related Pathways:

  Regulation of Microtubule Dynamics, Epigenetic Writers and Erasers of Histones H2A H2B and H4

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  PAK2 (R186) polyclonal antibody detects endogenous levels of PAK2 protein.

• Applications Notes:

  Western blot (WB) analysis of PAK2 (R186) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:The spleen tissue lysate of Mouse (40ug) Lane3:The spleen tissue lysate of Rat (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4