ICAM-1 (Q506) polyclonal antibody

• Background:

  ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase in inflammation. Also, ICAM1 is detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54; ICAM1

• Swiss Prot:

  P05362

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 471-520 of Human ICAM-1.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 89, 92 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  ICAM-1 (Q506) polyclonal antibody detects endogenous levels of ICAM-1 protein.

• Applications Notes:

  Western blot (WB) analysis of ICAM-1 (Q506) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:A549 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:SP2/0 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4