TFIIE-β (K183) polyclonal antibody

• Background:

  The general transcription factor TFIIE consists of two subunits, α and β, and plays essential roles in transcription.Structure-function studies indicate that TFIIE has three-winged helix (WH) motifs, with one in TFIIEα and two in TFIIEβ. by binding to the clamp region of RNA polymerase II, TFIIEα-WH promotes the conformational change that transforms the promoter-bound inactive preinitiation complex to the active complex.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; GTF2E2; TF2E2

• Swiss Prot:

  P29084

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthetic peptide, corresponding to amino acids 153-213 of Human TFIIE-β.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 33 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  TFIIE-β (K183) pAb detects endogenous levels of TFIIE-β protein.

• Applications Notes:

  Western blot (WB) analysis of TFIIE-β (K183) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Mouse (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4