RPA2 (A17) polyclonal antibody

• Background:

  RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Replication protein A 32 kDa subunit; RP-A p32; RF-A protein 2; RPA2; REPA2; RPA32; RPA34

• Swiss Prot:

  P15927

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthetic peptide, corresponding to the N-terminus of Human RPA2.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 32 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  RPA2 (A17) polyclonal antibody detects endogenous levels of RPA2 protein.

• Applications Notes:

  Western blot (WB) analysis of RPA2 (A17) pAb at 1:500 dilution Lane1:H1792 whole cell lysate (40ug) Lane2:EC9706 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:HCT116 whole cell lysate (40ug) Lane6:PMVEC whole cell lysate (40ug) Lane7:3T3-L1 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4