IRAK1 (T381) polyclonal antibody

• Background:

  Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1α and IL-1β, and a specific receptor antagonist, IL-1Rα. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway. The IL-1-dependent kinase termed IRAK (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFκB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Interleukin-1 receptor-associated kinase 1; IRAK-1; IRAK1; IRAK

• Swiss Prot:

  P51617

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 350-400 of Human IRAK1.

• Conjugation:

  Unconjugated

• Related Pathways:

  Toll-like Receptor signaling

• Applications:

  WB IF

• Dilution:

  WB: 1:500~1:1000 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 76 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IRAK1 (T381) polyclonal antibody detects endogenous levels of IRAK1 protein.

• Applications Notes:

  Western blot (WB) analysis of IRAK1 (T381) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4