PIASy (D488) polyclonal antibody

• Background:

  The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  E3 SUMO-protein ligase PIAS4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma; PIASy; PIAS4; PIASG

• Swiss Prot:

  Q8N2W9

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 455-506 of Human PIASy.

• Conjugation:

  Unconjugated

• Related Pathways:

  Jak Stat : IL-6 Receptor Signaling

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 56 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  PIAS4 (D488) polyclonal antibody detects endogenous levels of PIAS4 protein.

• Applications Notes:

  Western blot (WB) analysis of PIASy (D488) pAb at 1:1000 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (20ug) Lane4:HEK293T whole cell lysate (10ug) Lane5:K562 whole cell lysate (10ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4