EEF2K (P360) polyclonal antibody

CAT: 0384-BS1100-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS1100-01Size:50 µL
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Background
EEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K
Swiss Prot
O00418
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 331-380 of Human eEF2K.
Conjugation
Unconjugated
Related Pathways
Signaling Pathways Activating p38 MAP Kinase
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 105 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EEF2K (P360) polyclonal antibody detects endogenous levels of eEF2K protein.
Applications Notes
Western blot (WB) analysis of eEF2K (P360) polyclonal antibody at 1:1000 dillution Lane1:Hela whole cell lysate (40μg) Lane2:HCT-116 whole cell lysate (40μg) Lane3:C6 whole cell lysate (60μg)
Host or Source
Rabbit

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