EEF2 (A50) polyclonal antibody

CAT: 0384-BS1099-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS1099-01Size:50 µL
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Background
Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Elongation factor 2; EF-2; EEF2; EF2
Swiss Prot
P13639
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 20-60 of Human eEF2.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 95 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EEF2 (A50) polyclonal antibody detects endogenous levels of eEF2 protein.
Applications Notes
Western blot (WB) analysis of eEF2 (A50) polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Mouse kidney tissue lysate Lane3:Rat kidney tissue lysate
Host or Source
Rabbit

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