EEF2 (A50) polyclonal antibody

• Background :

  Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Elongation factor 2; EF-2; EEF2; EF2

• Swiss Prot :

  P13639

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 20-60 of Human eEF2.

• Conjugation :

  Unconjugated

• Applications :

  WB IHC IF

• Dilution :

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 95 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  EEF2 (A50) polyclonal antibody detects endogenous levels of eEF2 protein.

• Applications Notes :

  Western blot (WB) analysis of eEF2 (A50) polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Mouse kidney tissue lysate Lane3:Rat kidney tissue lysate

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4