EEF2 monoclonal antibody

CAT: 0384-MB66520-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-MB66520-01Size:50 µL
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Background
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A) . Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
EF2; Elongation factor 2; EF-2
Swiss Prot
P13639
Reactivity
Human
Immunogen
Purified recombinant human eEF2 protein fragments expressed in E.coli.
Conjugation
Unconjugated
Related Pathways
AMPK Signaling Pathway
Applications
WB, IF/ICC, ChIP
Dilution
WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), ChIP (1/10 - 1/50)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 95 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of EEF2 protein.
Applications Notes
Western blot analysis of EEF2 expression in HL60 (A), Jurkat (B), SHSY5Y (C), U20S (D), Hela (E) whole cell lysates.
Host or Source
Mouse

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