EEF2 monoclonal antibody

• Background:

  Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A) . Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  EF2; Elongation factor 2; EF-2

• Swiss Prot:

  P13639

• Reactivity:

  Human

• Immunogen:

  Purified recombinant human eEF2 protein fragments expressed in E.coli.

• Conjugation:

  Unconjugated

• Related Pathways:

  AMPK Signaling Pathway

• Applications:

  WB, IF/ICC, ChIP

• Dilution:

  WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), ChIP (1/10 - 1/50)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 95 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of EEF2 protein.

• Applications Notes:

  Western blot analysis of EEF2 expression in HL60 (A), Jurkat (B), SHSY5Y (C), U20S (D), Hela (E) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4