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HSL (P548) polyclonal antibody

CAT: 0384-AP0372-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-AP0372-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS
Swiss Prot
Q05469
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 520-570 of Human HSL.
Conjugation
Unconjugated
Related Pathways
Insulin &Glucose Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 85, 115 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HSL (P548) polyclonal antibody detects endogenous levels of HSL protein.
Applications Notes
Western blot analysis of HSL expression in Panc1 (A), SKOVCAR3 (B), C6 (C), 3T3L1 (D) whole cell lysates.
Host or Source
Rabbit

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