PKC θ (phospho-T538) polyclonal antibody

• Background:

  Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types. PKC theta is a member of the PKC family, and its predominant expression in hematopoietic cells suggests that it may play a role in signal transduction and growth regulatory pathways unique to these cells. PKC theta is a Ca (2+) -independent PKC isoform that is selectively expressed in T lymphocytes (and muscle), and it is thought to play an important role in T cell receptor-induced activation. Among several PKC isoenzymes expressed in T cells, PKC theta is unique in being rapidly recruited to the site of TCR clustering. It has been shown that PKC theta is essential for TCR-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Protein kinase C theta type; nPKC-theta; PRKCQ; PRKCT

• Swiss Prot:

  Q04759

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human PKC θ around the phosphorylation site of Threonine 538.

• Conjugation:

  Unconjugated

• Related Pathways:

  Protein Kinase C Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

• Applications:

  WB

• Dilution:

  WB:1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 80 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  PKC θ (phospho-T538) polyclonal antibody detects endogenous levels of PKC θ protein only when phosphorylated at Thr538.

• Applications Notes:

  Western blot (WB) analysis of PKC θ (phospho-T538) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:MCF-7 treated with LPS (100ng/ml) for 5 minutes whole cell lysate Lane3:MCF-7 treated with LPS (100ng/ml) for 30 minutes whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4