PKC theta (Phospho-T538) polyclonal antibody

• Background:

  Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction . PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters . Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occursin vivoat Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 . Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKC (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization . PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  PRKCT; Protein kinase C theta type; nPKC-theta

• Swiss Prot:

  Q04759

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKC theta with a phosphorylation site at T538. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  Regulation of Apoptosis, ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Protein Kinase C Signaling

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/200)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 78 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of PKC theta with a phosphorylation site at T538 protein.

• Applications Notes:

  Western blot analysis of PKC theta (pT538) expression in mouse muscle (A), rat muscle (B) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4