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COXI polyclonal antibody

CAT: 0384-BS1636-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1636-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Cytochrome c oxidase subunit I, COX1 (also designated COI, MTCO1 or oxidative phosphorylation (OxPhos) Complex IV, subunit I) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cytochrome c oxidase subunit 1; Cytochrome c oxidase subunit I; Cytochrome oxidase subunit 1; mt-Co1; Co1; COX1; COXI
Swiss Prot
Q9MD68
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to amino acids 433-445 of Mouse COXI.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 57 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
COXI polyclonal antibody detects endogenous levels of COXI protein.
Applications Notes
Western blot (WB) analysis of COXI pAb at 1:2000 dilution Lane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Skin tissue lysate of Rat (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug) Lane6:A375 whole cell lysate (30ug)
Host or Source
Rabbit

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