Products for Research Use Only

IgA (Y276) polyclonal antibody

CAT: 0384-BS3526-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS3526-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ig alpha-1 chain C region; IGHA1; Ig alpha-2 chain C region; IGHA2
Swiss Prot
P01876/P01877
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 241-290 of Human IgA.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgA (Y276) polyclonal antibody detects endogenous levels of IgA protein.
Applications Notes
Western blot (WB) analysis of IGHA1/IGHA2 pAb at 1:1000 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug)
Host or Source
Rabbit