M-RIP polyclonal antibody

CAT: 0384-CAS7516-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-CAS7516-01Size:50 µL
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip; MPRIP
Swiss Prot
Q6WCQ1
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human M-RIP.
Conjugation
Unconjugated
Applications
WB IP IHC
Dilution
WB: 1:5000~1:10000 IP:1:2000~1:8000 IHC:1:50~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 130-160kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
M-RIP polyclonal antibody detects endogenous levels of M-RIP protein.
Applications Notes
Western blot (WB) analysis of M-RIP polyclonal antibody at 1:5000 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:CT-26 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug) Lane4:Myla2059 whole cell lysate (40ug) Lane5:PC3 whole cell lysate (40ug) Lane6:SGC7901 whole cell lysate (40ug)
Host or Source
Rabbit

Related Products

CatalogName
BS91172-01RIP polyclonal antibody
BS80503-01RIPK1/RIP polyclonal antibody
BS62404-01P116 Rip polyclonal antibody
MB11533RIP monoclonal antibody
MB11534RIP monoclonal antibody
BS49491-01RIP Recombinant Rabbit mAb
BS45972-01RIP Recombinant Rabbit mAb
BS45237RIP Recombinant Rabbit mAb
PPL00506-3aPPLK/GFP+Puro-RIP shRNA-1
BS76771-01Neurofilament M polyclonal antibody

Alternative Products

CatalogName
MBS8523819-01M-RIP Polyclonal Antibody
MBS9512369-01M-RIP Rabbit Polyclonal Antibody
APR00057GPolyclonal RIP Antibody
MBS668767-01Polyclonal Antibody to RIP
E43S91172RIP polyclonal antibody
PAV9038Rabbit Anti-M-RIP/MPRIP Polyclonal Antibody
BT-AP12294-01RIP Rabbit Polyclonal Antibody
C21091-01M-RIP Antibody
STJ95513Anti-M-RIP antibody
sc-149201-PRM-RIP (m) -PR

Popular Products