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WNT16 polyclonal antibody

CAT: 0384-BS62537-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS62537-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The Wnt genes encode a family of secreted extracellular signaling glycoproteins, which function in a variety of important developmental processes such as regulation of cell growth and differentiation. Wnt proteins also play roles in carcinogenesis. Wnt-14, rather than Wnt-15, is preferentially expressed in various types of human cancer. Wnt-15 is expressed in fetal and adult kidney and is most homologous to Wnt-14. Wnt-16, another member in the Wnt family, has two mRNA isoforms, Wnt-16a and Wnt-16b. These isoforms differ in the composition of their 5'UTR and first exon, which results in differential expression. Wnt-16a is expressed only in pancreas, whereas Wnt-16b is highly expressed in adult kidney, placenta, brain, heart and spleen, but not in bone marrow. However, Wnt-16 transcripts are present in bone marrow and cell lines derived from pre-B acute lymphoblastoid leukemia patients carrying the E2A-Pbx1 hybrid gene. Thus, Wnt-16 is a downstream target of E2APbx1, and the Wnt-16-mediated autocrine growth mechanism may contribute to the development of t (1;19) pre-B acute lymphoblastoid leukemias.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein Wnt-16; WNT16
Swiss Prot
Q9UBV4
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human WNT16.
Conjugation
Unconjugated
Applications
WB
Dilution
WB:1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
WNT16 polyclonal antibody detects endogenous levels of WNT16 protein.
Applications Notes
Western blot (WB) analysis of WNT16 polyclonal antibody at 1:500 dilution LaneA:SP2/0 whole cell lysate LaneB:The Spleen tissue lysate of Mouse LaneC:The Spleen tissue lysate of Rat LaneD:HEK293T whole cell lysate LaneE:Panc1 whole cell lysate
Host or Source
Rabbit