SPAK polyclonal antibody

CAT: 0384-BS62488-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS62488-01Size:50 µL
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Background
SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitrostudies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response. Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3. WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380. Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315. Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity. SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39; STK39; SPAK
Swiss Prot
Q9UEW8
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human SPAK.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB:1:500~1:1000 IHC:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 70 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SPAK polyclonal antibody detects endogenous levels of SPAK protein.
Applications Notes
Western blot (WB) analysis of SPAK polyclonal antibody at 1:500 dilution LaneA:CT26 whole cell lysate LaneB:PC12 whole cell lysate LaneC:A549 whole cell lysate LaneD:MCF-7 whole cell lysate
Host or Source
Rabbit

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