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ENOS (Phospho-S615) polyclonal antibody

CAT: 0384-BS64540-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS64540-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS) ; inducible NOS (iNOS) or macrophage NOS (mNOS) ; and endothelial NOS (eNOS) .eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3
Swiss Prot
P29474
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Serine 615.
Conjugation
Unconjugated
Related Pathways
Hypoxia Signaling, Tumor Angiogenesis
Applications
WB
Dilution
WB:1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 140 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ENOS (Phospho-S615) polyclonal antibody detects endogenous levels of eNOS protein only when phosphorylated at Ser615.
Applications Notes
Western blot (WB) analysis of eNOS (Phospho-S615) polyclonal antibody at 1:500 dilution LaneA:K562 whole cell lysate LaneB:The Spleen tissue lysate of Mouse LaneC:The Spleen tissue lysate of Rat
Host or Source
Rabbit

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