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Phospho-eNOS (Thr113) Polyclonal Antibody

CAT: 0498-bs-3589RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-3589RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.
Synonyms
ENOS; Nos-3; ecNOS; 2310065A03Rik; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; Nos3
Gene ID
18127
Swiss Prot
P70313
Modification Site
Thr113
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-eNOS (Thr113)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113
Applications
WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Specificity
This phosphorylation site is homologous across the listed species; this phosphorylation site does not exist in human due to a Thr-Ser substitution.
Gene ID URL
18127
Predicted Cross Reactivity
Mouse, Rat, Dog, Rabbit

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