Histone H3 (MonoMethyl-R8) polyclonal antibody

• Background:

  In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Hist1h3a; Hist1h3b; H3 histone family; member A; H3/A; H31; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1; H3a; Histone cluster 1; H3a; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

• Swiss Prot:

  P68431/Q71DI3/P84243/Q6NXT2

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to Human Histone H3.

• Conjugation:

  Unconjugated

• Related Pathways:

  Histone Lysine Methylation Pathway, Examples of Crosstalk Between Post-translational Modifications, DNA Methylation

• Applications:

  WB

• Dilution:

  WB:1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Methylated

• Molecular Weight:

  ~ 17 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Histone H3 (MonoMethyl-R8) polyclonal antibody detects endogenous levels of Histone H3 protein.

• Applications Notes:

  Western blot (WB) analysis of Histone H3 (MonoMethyl-R8) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:H1792 whole cell lysate LaneC:A549 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4