RNF14 polyclonal antibody

CAT: 0384-BS65257-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS65257-01Size:50 µL
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Background
Ring finger protein 14 (RNF14) Homo sapiens The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein
Swiss Prot
Q9UBS8
Reactivity
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human RNF14. AA range:361-410
Conjugation
Unconjugated
Applications
WB, ELISA
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For
Specificity
ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein.
Applications Notes
Western blot analysis of lysates from RAW264.7 cells, using RNF14 Antibody. The lane on the right is blocked with the synthesized peptide.
Host or Source
Rabbit

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