RNF14 polyclonal antibody

• Background :

  Ring finger protein 14 (RNF14) Homo sapiens The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]

• CAS Number :

  9007-83-4

• Structure Composition :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Product Name Alternative :

  RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein

• Swiss Prot :

  Q9UBS8

• Reactivity :

  Human

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human RNF14. AA range:361-410

• Conjugation :

  Unconjugated

• Applications :

  WB, ELISA

• Dilution :

  Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Modification :

  Unmodification

• Molecular Weight :

  ~ 50 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For

• Specificity :

  ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein.

• Applications Notes :

  Western blot analysis of lysates from RAW264.7 cells, using RNF14 Antibody. The lane on the right is blocked with the synthesized peptide.

• Host or Source :

  Rabbit