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MAP2K7 (Ab-271) polyclonal antibody

CAT: 0384-BS65255-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65255-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mitogen-activated protein kinase kinase 7 (MAP2K7) Homo sapiens The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
MAP2K7; JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; S
Swiss Prot
O14733
Reactivity
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Ser271. AA range:236-285
Conjugation
Unconjugated
Related Pathways
SAPK JNK Signaling Cascades
Applications
WB, IHC-p, IF (paraffin section), ELISA
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Molecular Weight
~ 47 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For
Specificity
Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271.
Applications Notes
Western Blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody
Host or Source
Rabbit