MRPS32 polyclonal antibody

• Background:

  Mitochondrial ribosomal protein L42 (MRPL42) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provid

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Product Name Alternative:

  MRPL42; MRPL31; MRPS32; RPML31; HSPC204; PTD007; 39S ribosomal protein L42; mitochondrial; L42mt; MRP-L42; 28S ribosomal protein S32, mitochondrial; MRP-S32; S32mt; 39S ribosomal protein L31, mitochondrial; L31mt; MRP-L31

• Swiss Prot:

  Q9Y6G3

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human MRPS42. AA range:75-124

• Conjugation:

  Unconjugated

• Applications:

  IHC-p, IF (paraffin section), ELISA

• Dilution:

  Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Modification:

  Unmodification

• Molecular Weight:

  /

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  MRP-S32 Polyclonal Antibody detects endogenous levels of MRP-S32 protein.

• Applications Notes:

  Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100 (4° overnight) . High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4