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Phospho-KLF5 (Ser311) polyclonal antibody

CAT: 0384-BS65572-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65572-01Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
KLF5 (phospho Ser311) ; KLF5 (phospho S311) ; Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5; KLF5_HUMAN.
Swiss Prot
Q13887
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
Immunogen
KLH conjugated synthesised phosphopeptide derived from human KLF5 around the phosphorylation site of Ser311:PG (p-S) PD
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Purity
Affinity purified by Protein A
Modification
Phosphorylation
Molecular Weight
~50kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-KLF5 (Ser311) polyclonal antibody detects endogenous levels of KLF5 protein only when phosphorylated at Ser311.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5 (Ser311) ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Host or Source
Rabbit

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