Phospho-KLF5 (Ser311) polyclonal antibody

• Background:

  Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5.

• Structure Composition:

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative:

  KLF5 (phospho Ser311) ; KLF5 (phospho S311) ; Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5; KLF5_HUMAN.

• Swiss Prot:

  Q13887

• Reactivity:

  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

• Immunogen:

  KLH conjugated synthesised phosphopeptide derived from human KLF5 around the phosphorylation site of Ser311:PG (p-S) PD

• Conjugation:

  Unconjugated

• Applications:

  IHC-P

• Dilution:

  IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

• Purity:

  Affinity purified by Protein A

• Modification:

  Phosphorylation

• Molecular Weight:

  ~50kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Phospho-KLF5 (Ser311) polyclonal antibody detects endogenous levels of KLF5 protein only when phosphorylated at Ser311.

• Applications Notes:

  Paraformaldehyde-fixed, paraffin embedded (Mouse placenta) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5 (Ser311) ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4