FXR polyclonal antibody

• Background:

  The farnesoid X receptor (FXR/NR1H4) is a member of the nuclear hormone receptor superfamily and is a master regulator of bile acid synthesis. FXR/NR1H4 heterodimerizes with RXR-alpha upon activation by bile acids, which begins a regulatory cascade involving SHP and LRH-1 to control lipid homeostasis. FXR/NR1H4 has also been shown to be a critical regulator of glucose homeostasis. In addition to directly regulating genes, FXR/NR1H4 also plays a post transcriptional role in bile acid metabolism by transcribing the RNA-binding protein ZFP36L1, which in turn downregulates the key enzyme Cyp7a1. Mutations in human FXR/NR1H4 have been shown to cause cholestasis and liver disease in neonatal patients. FXR/NR1H4 can also control Lgr5+ intestinal stem cell proliferation and its upregulation has been shown to inhibit colorectal cancer progression. Agonists against FXR/NR1H4 are being evaluated for various liver diseases and diabetes.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Bile acid receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14; NR1H4; BAR; FXR; HRR1; RIP14

• Swiss Prot:

  Q96RI1

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding to Human FXR.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:1000~1:2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 67 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  FXR polyclonal antibody detects endogenous levels of FXR protein.

• Applications Notes:

  Western blot (WB) analysis of FXR polyclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4