Stat3 polyclonal antibody

• Background:

  Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF

• Swiss Prot:

  P40763

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein.

• Conjugation:

  Unconjugated

• Related Pathways:

  Immune Cell Signaling, Jak Stat : IL-6 Receptor Signaling

• Applications:

  WB IP IF

• Dilution:

  WB:1:500~1:1000 IP: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 80,89KDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  STAT3 polyclonal antibody detects endogenous levels of STAT3 protein.

• Applications Notes:

  Western blot (WB) analysis of Stat3 pAb at 1:1000 dilution Lane1:H9C2 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:THP-1 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4