Stat1 polyclonal antibody

CAT: 0384-AP0364-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-AP0364-01Size:50 µL
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Background
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs) . Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1; STAT1α; STAT1β
Swiss Prot
P42224
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 680-720 of Human Stat1.
Conjugation
Unconjugated
Related Pathways
Immune Cell Signaling, Jak Stat : IL-6 Receptor Signaling, Signaling Pathways Activating p38 MAP Kinase
Applications
WB IHC IF IP
Dilution
WB:1:500~1:1000 IF: 1:50~1:200 IHC:1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 87 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Stat1 polyclonal antibody detects endogenous levels of Stat1 protein.
Applications Notes
Western blot (WB) analysis of Stat1 polyclonal antibody at 1:1000 dillution Lane1:NIH-3T3) whole cell lysate (40μg) Lane2:NIH-3T3 treated with UV (4h) whole cell lysate (40μg) Lane3:NIH-3T3 treated with PMA (60ng/ml, 30min) whole cell lysate (40μg) Lane4:Hela whole cell lysate (40μg) Lane5:Hela treated with IFN-γ (100ng/ml,15min) whole cell lysate (40μg) Lane6:Hela treated with PMA (60ng/ml, 30min) whole cell lysate (40μg)
Host or Source
Rabbit

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