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Stat1 (Phospho-Y701) polyclonal antibody

CAT: 0384-AP0246-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-AP0246-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The Stat1 transcription factor is activated in response to a large number of ligands and is essential for responsiveness to IFN-α and IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding. Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa) . In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors. In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK) -dependent pathway in response to IFN-α and other cellular stresses. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1
Swiss Prot
P42224
Reactivity
Human, Mouse
Immunogen
Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Tyrosine 701.
Conjugation
Unconjugated
Related Pathways
ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway (, Inhibition of Apoptosis, Signaling Pathways Activating p38 MAP Kinase
Applications
WB IF IP
Dilution
WB 1:500~1:1000 IF: 1:100~1:500 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 84 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Stat1 (Phospho-Y701) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Tyr701.
Applications Notes
Western blot (WB) analysis of Stat1 (Phospho-Y701) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (30ug) Lane2:HeLa treated with IFN-α (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight whole cell lysate (30ug) Lane3:3T3-L1 whole cell lysate (30ug)
Host or Source
Rabbit

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