RPS6 (L234) polyclonal antibody

• Background:

  One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  40S ribosomal protein S6; Phosphoprotein NP33; Small ribosomal subunit protein Es6; RPS6; OK/SW-cl.2

• Swiss Prot:

  P62753

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Synthetic peptide, corresponding to Human RPS6.

• Conjugation:

  Unconjugated

• Related Pathways:

  MAPK Erk in Growth and Differentiation Pathway (, PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase

• Applications:

  WB IP

• Dilution:

  WB: 1:5000~1:10000 IP: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 32 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  RPS6 (L234) polyclonal antibody detects endogenous levels of RPS6 protein.

• Applications Notes:

  Western blot (WB) analysis of RPS6 (L234) pAb at 1:5000 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:The Liver tissue lysate of Mouse (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:Jurket whole cell lysate (40ug) Lane5:THP-1 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4