RPS6 monoclonal antibody

• Background:

  One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  40S ribosomal protein S6; Phosphoprotein NP33

• Swiss Prot:

  P62753

• Reactivity:

  Human, Mouse, Rat, Monkey

• Immunogen:

  Synthetic peptide derived from human S6 Ribosomal Protein.

• Conjugation:

  Unconjugated

• Related Pathways:

  PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase

• Applications:

  WB, IF/ICC, IP

• Dilution:

  WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 32 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of RPS6 protein.

• Applications Notes:

  Western blot analysis of RPS6 expression in Ramos (A), Jurkat (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4