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Raptor (S792) polyclonal antibody

CAT: 0384-AP1001-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-AP1001-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Regulatory associated protein of FRAP, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (FRAP), and is essential for FRAP signalling in vivo. Raptor binding to FRAP is critical for FRAP-catalysed substrate phosphorylation of 4E-BP1. The raptor-FRAP complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of FRAP kinase activity under nutrient-deprived conditions and is an important component in the FRAP pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR) -scaffold protein; RPTOR; KIAA1303; RAPTOR
Swiss Prot
Q8N122
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding Human Raptor.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, PI3K AKT signaling, Insulin &Glucose Signaling, Glutamine Metabolism, MTOR signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 150 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Raptor (S792) polyclonal antibody detects endogenous levels of Raptor protein.
Applications Notes
Western blot (WB) analysis of Raptor (Ser792) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug)
Host or Source
Rabbit

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