PPM1A polyclonal antibody

• Background :

  The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA; PPM1A; PPPM1A

• Swiss Prot :

  P35813

• Reactivity :

  Mouse, Rat

• Immunogen :

  KLH conjugated synthetic peptide derived from human PP2CA/PPM1A: 101-200/382

• Conjugation :

  Unconjugated

• Applications :

  WB IHC IF ELISA

• Dilution :

  WB 1:500 - 1:2000 IHC 1:100 - 1:500 IF 1:100 - 1:500 ELISA 1:5000 - 1:10000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 42 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  PPM1A polyclonal antibody detects endogenous levels of PPM1A protein.

• Applications Notes :

  Paraformaldehyde-fixed, paraffin embedded (Rat brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPM1A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

• Host or Source :

  Rabbit