Centrin-2 polyclonal antibody

• Background:

  Centrins are small conserved microtubule nucleating proteins localized to centrosomes, also known as microtubule organizing centers (MTOC), in eukaryotic cells. Centrin-1 is associated with cells that have cilia and flagella, whereas centrin-2 and -3 are ubiquitously expressed and important in centrosome duplication during cell division, as well as the structure and function of the MTOC . Human centrin-2 has also been shown to localize to nuclear pores and to have a role in regulation of mRNA export . The yeast ortholog of centrin-2, CDC31, plays a part in control of protein degradation and sensitivity to DNA damage .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  CALT; CEN2; Centrin-2; Caltractin isoform 1

• Swiss Prot:

  P41208

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length protein of human Centrin-2

• Conjugation:

  Unconjugated

• Applications:

  WB, IF/ICC

• Dilution:

  WB (1/500 - 1/2000), IF/ICC (1/50 - 1/200)

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 20 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of Centrin-2 protein.

• Applications Notes:

  Western blot analysis of Centrin-2 expression in HT29 (A), MCF7 (B), mouse spleen (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4