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Rock-2 polyclonal antibody

CAT: 0384-CAS75112-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-CAS75112-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains.The C-terminus of ROCK negatively regulates its kinase activity. Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase. This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Rho-associated protein kinase 2; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2; ROCK2
Swiss Prot
O75116
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human Rock-2.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, Regulation of Microtubule Dynamics
Applications
WB IP
Dilution
WB: 1:5000~1:10000 IP:1:2000~1:8000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 180kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Rock-2 polyclonal antibody detects endogenous levels of Rock-2 protein.
Applications Notes
Western blot (WB) analysis of Rock-2 polyclonal antibody at 1:10000 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:The brain tissue lysate of Rat (40ug) Lane3:The brain tissue lysate of Mouse (40ug) Lane4:U-87MG whole cell lysate (40ug) Lane5:H1792 whole cell lysate (40ug) Lane6:SGC7901 whole cell lysate (40ug)
Host or Source
Rabbit

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