WASP (phospho-Y290) polyclonal antibody

• Background:

  Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905) .Important for efficient actin polymerization (PubMed:8625410, PubMed:12235133, PubMed:16275905) .Possible regulator of lymphocyte and platelet function (PubMed:9405671) .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  IMD2; Wiskott-Aldrich syndrome protein; WASp

• Swiss Prot:

  P42768

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human WASP (pY290) . The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC, FC

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 70 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of WASP (pY290) protein.

• Applications Notes:

  Western blot analysis of WASP (pY290) expression in Jurkat (A), SHSY5Y (B), mouse spleen (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4