MRPL40 polyclonal antibody

• Background :

  Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Deletions in this gene may contribute to the etiology of velo-cardio-facial syndrome and DiGeorge syndrome. [provided by RefSeq, Jul 2008]

• Structure Composition :

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative :

  NLVCF; URIM; 39S ribosomal protein L40 mitochondrial; L40mt; MRP-L40; Nuclear localization signal-containing protein deleted in velocardiofacial syndrome; Up-regulated in metastasis

• Swiss Prot :

  Q9NQ50

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRPL40. The exact sequence is proprietary.

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC

• Dilution :

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 25 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Recognizes endogenous levels of MRPL40 protein.

• Applications Notes :

  Western blot analysis of MRPL40 expression in mouse muscle (A), mouse liver (B), rat muscle (C) whole cell lysates.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4