USP30 polyclonal antibody

• Background:

  Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN) : hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951) .Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling (PubMed:25621951) .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  Ubiquitin carboxyl-terminal hydrolase 30; Deubiquitinating enzyme 30; Ubiquitin thioesterase 30; Ubiquitin-specific-processing protease 30; Ub-specific protease 30

• Swiss Prot:

  Q70CQ3

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human USP30. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB, IF/ICC

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 58 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of USP30 protein.

• Applications Notes:

  Western blot analysis of USP30 expression in MCF7 (A), A549 (B), U2OS (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4