CD27 polyclonal antibody

• Background:

  CD27 (TNFRSF7) is a transmemebrane protein of the TNF receptor superfamily (TNFRSF) . It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice) . CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regulatory (Breg) cells. It is constitutively expressed on naïve CD4 and CD8 T cells and its expression is further upregulated upon T cell activation. CD27 is one of the two most important co-stimulatory receptors for T cell priming (the other one is CD28) . While CD28 co-stimulatory signal mainly triggers cell proliferation, CD27 co-stimulatory signal primarily promotes cell survival and differentiation. Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR associated factors (TRAFs), the adaptor molecules that are associated with CD27 cytoplasmic tail domain. Upon activation CD27 is shed from cell surface and soluble CD27 is used as a marker of T cell activation.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD27; TNFRSF7

• Swiss Prot:

  P26842

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Recombinant protein of human CD27.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:1000~1:2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 65 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD27 polyclonal antibody detects endogenous levels of CD27 protein.

• Applications Notes:

  Western blot (WB) analysis of CD27 polyclonal antibody at 1:1000 dilution Lane1:EC9706 cell membrane lysate (24ug) Lane2:A549 whole cell lysate (30ug) Lane3:SGC7901 whole cell lysate (30ug) Lane4:The Heart tissue lysate of Mouse (30ug) Lane5:The Kidney tissue lysate of Rat (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4