CD275 polyclonal antibody

• Background:

  Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity) .

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD_antigen: CD275; ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653

• Swiss Prot:

  O75144

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant protein of human CD275.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:1000~1:2000 IF 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 70 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD275 polyclonal antibody detects endogenous levels of CD275 protein.

• Applications Notes:

  Western blot (WB) analysis of CD275 polyclonal antibody at 1:1000 dilution Lane1:L02 whole cell lysate (30ug) Lane2:SP2/0 cell membrane lysate (24ug) Lane3:HEK293T whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4