ADAMTS7 polyclonal antibody

• Background:

  ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 modules) is a family of zinc-dependent proteases that are implicated in a variety of normal and pathological conditions, including arthritis and cancer. ADAMTS protein family members contain an N-terminal propeptide domain, a metalloproteinase domain, a disintegrin-like domain and a C-terminus that contains a varying number of thrombospondin type-1 (TSP-1) motifs. ADAMTS genes are primarily expressed in fetal tissues, including lung, kidney and liver. ADAMTS-7 (ADAM metallopeptidase with thrombospondin type 1 motif, 7), also known as COMPase, is a 1,686 amino acid protein that exists as two alternatively spliced isoforms. Encoded by a gene that maps to human chromosome 15q25.1, ADAMTS-7 contains eight TSP-1 motifs and binds one zinc ion per subunit. ADAMTS-7 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ADAMTS-7 is also located in meniscus, bone, tendon, cartilage, synovium, fat and ligaments, and is up-regulated in articular cartilage and synovium in arthritis patients. ADAMTS-7 functions as a metalloprotease and may play a role in the degradation of COMP. ADAMTS-7 is pH dependent, with optimum pH between 7.5 and 9.5.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  A disintegrin and metalloproteinase with thrombospondin motifs 7 (EC:3.4.24.-) ; ADAM-TS 7; ADAM-TS7; ADAMTS-7; COMPase; ADAMTS7

• Swiss Prot:

  Q9UKP4

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding to Human ADAMTS7.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:1000~1:2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 230 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  ADAMTS7 polyclonal antibody detects endogenous levels of ADAMTS7 protein.

• Applications Notes:

  Western blot (WB) analysis of ADAMTS7 polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate (30ug) Lane2:EC9706 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4