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PCNA monoclonal antibody

CAT: 0384-MB66679-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-MB66679-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
Proliferating cell nuclear antigen; PCNA; Cyclin
Swiss Prot
P12004
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Immunogen
Purified recombinant human PCNA protein fragments expressed in E.coli.
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC, IP
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 36 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of PCNA protein.
Applications Notes
Western blot analysis of PCNA expression in Hela (A), NIH3T3 (B), COS7 (C), C6 (D), CHOK1 (E) whole cell lysates.
Host or Source
Mouse

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