PCNA Monoclonal Antibody

• Background:

  Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

• Abbreviation:

  PCNA

• UniProt:

  P12004

• Host:

  Mouse

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Recombinant human PCNA protein expressed by E.coli

• Target:

  Proliferating cell nuclear antigen, PCNA, Cyclin

• Clonality:

  Monoclonal

• Clone:

  9C6

• Conjugation:

  Unconjugated

• Applications:

  WB; IP; IHC

• Field of Research:

  Cell Biology; Epigenetics and Nuclear Signaling; Tags & Cell Markers; Cancer; Isotype; Loading Controls

• Purification:

  Protein A/G Purification

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:2000-1:4000; IP 4ug/sample; IHC 1:200-1:400

• Buffer:

  PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

• Calculated Molecular Weight:

  29 kDa

• Observed Molecular Weight:

  33 kDa

• Isotype:

  IgG2a