Nucleophosmin monoclonal antibody

• Background:

  Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

• Swiss Prot:

  P06748

• Reactivity:

  Human, Mouse

• Immunogen:

  Purified recombinant full length of human NPM1 protein expressed in E.coli.

• Conjugation:

  Unconjugated

• Applications:

  WB, ChIP

• Dilution:

  WB (1/500 - 1/1000), ChIP (1/10 - 1/50)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 38 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of Nucleophosmin protein.

• Applications Notes:

  Western blot analysis of Nucleophosmin expression in Hela (A), Jurkat (B), NIH3T3 (C) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4