Nucleophosmin monoclonal antibody

• Background:

  Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486) . In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029) .

• Structure Composition:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

• Product Name Alternative:

  B23; NPM

• Swiss Prot:

  P06748

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide of human Nucleophosmin

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-P, IP

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

• Purity:

  Affinity Purified

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 33 kDa; Observed MW: 38 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of Nucleophosmin in HCT116, T47D, MCF-7 lysates using Nucleophosmin antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4