Phospho-MAPKAPK-2/MK2-T334 polyclonal antibody

• Background:

  This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  MAPKAPK2; MAPKAP-K2; MK-2; MK2

• Swiss Prot:

  P49137

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic phosphorylated peptide around T334 of human MAPKAPK2 (NP_116584.2?) .

• Conjugation:

  Unconjugated

• Applications:

  IHC

• Dilution:

  IHC,1:50 - 1:100

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylated

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Phosphorylated

• Applications Notes:

  Immunohistochemistry of paraffin-embedded Mouse kidney using Phospho-MAPKAPK-2/MK2-T334 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4