Phospho-Rb (Ser807) polyclonal antibody

• Background :

  Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

• Structure Composition :

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

• Product Name Alternative :

  RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

• Swiss Prot :

  P06400

• Reactivity :

  Human, Rat

• Immunogen :

  A synthetic phosphopeptide corresponding to residues surrounding Ser807 of human Rb

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC-F, IHC-P, ICC/IF

• Dilution :

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

• Purity :

  Affinity Purified

• Modification :

  Phosphorylated

• Molecular Weight :

  Calculated MW: 106 kDa; Observed MW: 110 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  IgG

• Applications Notes :

  Western blot analysis of Rb in CHO-K1, C6 lysates using Phospho-Rb antibody.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4